About Us

Database Organization of the RTCGD

The data of Retrovirus Integration Sites (RISs) in the RTCGD is obtained from using a high-throughput inverse PCR method or splinkerette method. We cloned and sequenced independent RISs from tumor and then added these sequences to RTCGD. Then, by using public UCSC mouse genome server, we positioned these RIS sequences in the mouse genome and identified candidate genes.

In addition to retrovirus tagging, Sleeping Beauty (SB) transposon systems are used for mouse mutagenesis. In RTCGD, users can also search the results of cancer-gene screening by SB transposon system. The information about germline mutagenesis by SB transposon system is stored on NCI's transposon database.

The RTCGD Database consists from following sections:

1. Retrovirus Tagged Cancer Gene (mm8):
   Database for retrovirus and transposon insertion sites (annotation by UCSC Mouse Assembly March, 2006)
   • Easy Search: Search tool based on gene symbol or gene name
   • Model Search: Search tool based on tumor models and/or tumor types
   • Interaction Search: Search tool for gene-to-gene interaction
   • Help: Frequently Asked Questions
   • About us:
     • Site Map: Database organization
     • References: References about retrovirus tagging and transposon mutagenesis
     • Links: Useful resources
2. Retrovirus Tagged Cancer Gene Database (mm7):
   Database for retrovirus and transposon insertion sites (annotation by UCSC Mouse Assembly Aug, 2005)

Development of the RTCGD

• Dr. Neal Copeland developed the database with support from the following labs.
  
  
  • Institute of Molucular and Cellular Biology in Singapore
    • Neal G. Copeland Lab
    • Nancy A. Jenkins Lab
  • Netherlands Cancer Institute
    • Division of Molecular Genetics: Anton Berns Lab
    • Division of Molecular Genetics: Maarten van Lohuizen Lab
  • Albert Einstein College of Medicine
    • Department of Molecular Genetics: Jack Lenz Lab
• To cite the use of the RTCG Database from this website in a publication, please quote the following:
  1. stable URL (http://RTCGD.ncifcrf.gov)
  2. RTCGD: retroviral tagged cancer gene database
     Nucl.Acids Res (2004) 32: Database Issue